Results of the Adult COVID-19 Lifestyle Matching Study.

Objective: The aim of our case-control study was to find the influence of lifestyle and comorbidities on COVID-19 susceptibility, identify risk factors and protective factors, and identify ways to encourage people to adopt a healthy lifestyle. Methods: Patients with COVID-19 were matched with non-COVID-19 participants in a ratio of 1:2. Univariate analysis was performed using the chi-square test, and multivariate analysis was performed using conditional logistic regression. Results: Multivariate analysis using conditional logistic regression found that alcohol consumption (AC) and a bland diet increased the risk of COVID-19, while college degrees and above, smoking, drinking tea, and exercise, especially walking, significantly reduced the risk of COVID-19. Conclusion: After removing the effects of demographic factors, the study demonstrated that AC significantly reduced the ability of the body to resist COVID-19 infection. Moreover, following a bland diet increased the susceptibility to COVID-19. Notably, people who drank tea and performed regular exercises, especially walking, were significantly less likely to be infected with COVID-19. College degree or above relative illiteracy is COVID-19 protective factors of infection.

In vitro selection of a single-strand DNA aptamer targeting the receptor-binding domain of SARS-CoV-2 spike protein.

Nucleic acid aptamers are developed from a pool of random oligonucleotide libraries with an in vitro selection through systematic evolution of ligands via exponential enrichment (SELEX) process, which are capable of specific and high-affinity molecular binding against targets. The receptor-binding domain (RBD) of spike protein from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is involved in the early stages of viral infection, is a promising target for aptamer selection. Currently, there are no effective approaches to prevent virus from spreading. In this study, a new ssDNA aptamer RBD/S-A1 binding to the RBD of spike protein from SARS-CoV-2 with high affinity (Kd=1.74 ± 0.2 nM) and low cross-binding activity was selected and evaluated. Although aptamers targeting the RBD of spike protein from SARS-CoV-2 have been described in a handful of previous studies, the RBD/S-A1 aptamer identified in this work may be considered as a potential supplementation for the current diagnosis and research of coronavirus SARS-CoV-2.

Effect of an online resourcefulness training in improving psychological well-being of front-line medical staff: a quasi-experimental study.

BACKGROUND: The global COVID-19 pandemic is still not under effective control, and strong workplace supports with comprehensive mental health interventions are urgently needed to help medical staff effectively respond to the pandemic. This study aimed to verify the effect of an online resourcefulness training program on the resourcefulness, and psychological variables of front-line medical staff working in the COVID-19 isolation ward. DESIGN: A pre-test and post-test quasi-experimental design with control group was employed. PARTICIPANTS: A total of 60 participants working in two isolation wards were recruited via convenience sampling. The two isolation wards were randomly assigned to the control group (isolation ward 1, n = 30) and the intervention group (isolation ward 2, n = 30). INTERVENTION: The participants were trained online by video conferences and WeChat. The control group received conventional training (e.g., psychological training, psychological counseling), while the intervention group received a 4-h online resourcefulness training. Both groups learned updated guidelines of COVID-19 simultaneously via video conference. The primary outcomes (resourcefulness, anxiety, depression and coping styles) and the secondary outcome (psychological resilience) were measured before intervention and three time points after intervention. RESULTS: After the intervention and one week after the intervention, the resourcefulness, resilience, and positive response scores of the participants in the intervention group were significantly higher than those of the control group. The anxiety and negative response scores in the intervention group were significantly lower than those of the control group (all p < 0.05). One month after the intervention, the scores of resourcefulness, tenacity, and positive response of the intervention group were higher than those of the control group (all p < 0.05). Repeated measures analysis of variance showed that the two groups of participants had statistically significant changes in the time-based effect and group-based effect in resourcefulness, resilience, anxiety scores and coping styles (p < 0.01). CONCLUSION: The results showed that our online resourcefulness training can significantly improve the resourcefulness, resilience, and positive response scores and effectively reduce anxiety and depression scores of front-line medical staff. This demonstrates that online resourcefulness training would be an effective tool for the psychological adjustment of front-line medical staff in fighting against COVID-19.

Station Location Optimization for the One-Way Carsharing System: Modeling and a Case Study

Carsharing is regarded as a new mode of transportation that can meet the diversity of travel demands. Carsharing systems have different operating modes, and one-way systems are more widely used since cars can be dropped off at any station. However, their planning involves a series of joint decisions regarding the number, size, and location of stations, as well as the fleet size. This paper develops a data-driven mixed-integer linear programming (MILP) model for planning one-way carsharing systems that consider the spatial distribution of demand and the interacting decisions between stations. The characteristics of existing stations and their spatiotemporal correlations are an important part of the model. To solve the MILP model, the extension of the Benders decomposition algorithm is adopted. The practicality of the proposed approach is demonstrated in a case study in Beijing, China. The results show that the existing planning of carsharing could result in a serious waste of resources. In contrast, the proposed method can obtain effective results in a reasonable time. The location results corresponding to a different rate of satisfied demand show that increasing the parking spots to improve the interaction between stations can effectively reduce the cost of operations. It should be noted that this paper only considers the benefit of operators. Future works will be carried out to optimize the one-way carsharing system by considering the benefits of operators, as well as the benefits of users and society. In addition, the impact of COVID-19 will be taken into account in future modeling and case studies.

Effect of virtual reality simulation training on the response capability of public health emergency reserve nurses in China: a quasiexperimental study.

OBJECTIVE: To develop a virtual reality simulation training programme, and further verify the effect of the programme on improving the response capacity of emergency reserve nurses confronting public health emergencies. DESIGN: A prospective quasiexperimental design with a control group. PARTICIPANTS: A total of 120 nurses were recruited and randomly divided into the control group and the intervention group. INTERVENTION: Participants underwent a 3-month training. The control group received the conventional training of emergency response (eg, theoretical lectures, technical skills and psychological training), while the intervention group underwent the virtual reality simulation training in combination with skills training. The COVID-19 cases were incorporated into the intervention group training, and the psychological training was identical to both groups. At the end of the training, each group conducted emergency drills twice. Before and after the intervention, the two groups were assessed for the knowledge and technical skills regarding responses to fulminate respiratory infectious diseases, as well as the capacity of emergency care. Furthermore, their pandemic preparedness was assessed with a disaster preparedness questionnaire. RESULTS: After the intervention, the scores of the relevant knowledge, the capacity of emergency care and disaster preparedness in the intervention group significantly increased (p<0.01). The score of technical skills in the control group increased more significantly than that of the intervention group (p<0.01). No significant difference was identified in the scores of postdisaster management in two groups (p>0.05). CONCLUSION: The virtual reality simulation training in combination with technical skills training can improve the response capacity of emergency reserve nurses as compared with the conventional training. The findings of the study provide some evidence for the emergency training of reserve nurses in better response to public health emergencies and suggest this methodology is worthy of further research and popularisation.

Expression and Possible Significance of ACE2 in the Human Liver, Esophagus, Stomach, and Colon.

Angiotensin-converting enzyme 2 (ACE2) has been identified as the key receptor of SARS coronavirus that plays a key role in the pathogenesis of SARS. It is known that ACE2 mRNA can be expressed in most organs. However, the protein expression of ACE2 is not clear yet. To explore the role of ACE2 as a precipitating factor in digestive organ damage in COVID-19, this study investigated the expression of ACE2 protein in the human liver, esophagus, stomach, and colon. The result showed that ACE2 can be expressed in the liver, esophagus, stomach, and colon, which suggests SARS-CoV-2 may enter the digestive system through ACE2 and cause liver damage and gastrointestinal damage. It is hoped that the result of the study will provide a new strategy for the prevention and treatment of digestive organ damage under COVID-19.

RBD-Modified Bacterial Vesicles Elicited Potential Protective Immunity against SARS-CoV-2.

The disease caused by SARS-CoV-2 infection threatens human health. In this study, we used high-pressure homogenization technology not only to efficiently drive the bacterial membrane to produce artificial vesicles but also to force the fusion protein ClyA-receptor binding domain (RBD) to pass through gaps in the bacterial membrane to increase the contact between ClyA-RBD and the membrane. Therefore, the load of ClyA-RBD on the membrane is substantially increased. Using this technology, we constructed a "ring-like" bacterial biomimetic vesicle (BBV) loaded with polymerized RBD (RBD-BBV). RBD-BBVs injected subcutaneously can accumulate in lymph nodes, promote antigen uptake and processing, and elicit SARS-CoV-2-specific humoral and cellular immune responses in mice. In conclusion, we evaluated the potential of this novel bacterial vesicle as a vaccine delivery system and provided a new idea for the development of SARS-CoV-2 vaccines.

COVID-19 combined with liver injury: Current challenges and management.

Coronavirus disease 2019 (COVID-19) combined with liver injury has become a very prominent clinical problem. Due to the lack of a clear definition of liver injury in patients with COVID-19, the different selection of evaluation parameters and statistical time points, there are the conflicting conclusions about the incidence rate in different studies. The mechanism of COVID-19 combined with liver injury is complicated, including the direct injury of liver cells caused by severe acute respiratory syndrome coronavirus 2 replication and liver injury caused by cytokines, ischemia and hypoxia, and drugs. In addition, underlying diseases, especially chronic liver disease, can aggravate COVID-19 liver injury. In the treatment of COVID-19 combined with liver injury, the primary and basic treatment is to treat the etiology and pathogenesis, followed by support, liver protection, and symptomatic treatment according to the clinical classification and severity of liver injury. This article evaluates the incidence, pathogenesis and prevention and treatment of COVID-19 combined with liver injury, and aims to provide countermeasures for the prevention and treatment of COVID-19 combined with liver injury.

Clinical characteristics of inpatients with coronavirus disease 2019 (COVID-19) in Sichuan province.

BACKGROUND: The outbreak of COVID-19 has resulted in serious concerns in China and abroad. To investigate clinical features of confirmed and suspected patients with COVID-19 in west China, and to examine differences between severe versus non-severe patients. METHODS: Patients admitted for COVID-19 between January 21 and February 11 from fifteen hospitals in Sichuan Province, China were included. Experienced clinicians trained with methods abstracted data from medical records using pre-defined, pilot-tested forms. Clinical characteristics between severe and non-severe patients were compared. RESULTS: Of the 169 patients included, 147 were laboratory-confirmed, 22 were suspected. For confirmed cases, the most common symptoms from onset to admission were cough (70·7%), fever (70·5%) and sputum (33·3%), and the most common chest CT patterns were patchy or stripes shadowing (78·0%); throughout the course of disease, 19·0% had no fever, and 12·4% had no radiologic abnormality; twelve (8·2%) received mechanical ventilation, four (2·7%) were transferred to ICU, and no death occurred. Compared to non-severe cases, severe ones were more likely to have underlying comorbidities (62·5% vs 26·2%, P = 0·001), to present with cough (92·0% vs 66·4%, P = 0·02), sputum (60·0% vs 27·9%, P = 0·004) and shortness of breath (40·0% vs 8·2%, P <  0·0001), and to have more frequent lymphopenia (79·2% vs 43·7%, P = 0·003) and eosinopenia (84·2% vs 57·0%, P = 0·046). CONCLUSIONS: The symptoms of patients in west China were relatively mild, and an appreciable proportion of infected cases had no fever, warranting special attention.

Anillin facilitates cell proliferation and induces tumor growth of hepatocellular carcinoma via miR-138/SOX4 axis regulation

Actin-binding protein Anillin plays a pivotal role in regulating cytokinesis during the cell cycle, and involves in tumorigenesis and progress. However, the exact regulation mechanism of Anillin in human hepatocellular carcinoma (HCC) remains largely unknown. In this study, we examined and verified the anomalous high expression of Anillin in both HCC patients'specimens and HCC cell lines. High expression of Anillin is associated with dismal clinicopathologic features of HCC patients and poor prognosis. We conducted loss-of and gain-of function studies in HCC Hep3B cells. Anillin presented a significantly facilitating effect on cell proliferation in vitro and induced remarkable tumor growth in vivo. We found that the over-expression of Anillin was driven by a potential axis of miR-138/SOX4. Transcription factor SOX4 presented a high expression profile positive correlated with Anillin, and ChIP assay validated the interaction between SOX4 and the specific sequence of the promoter region of Anillin gene. While, we verified miR-138 as an upstream regulator of SOX4, which is abrogated in HCC cells and exerts degenerating effect on SOX4 mRNA. In our conclusion, Anillin facilitates the cell proliferation and enhances tumor growth of HCC, and is modulated by miR-138/SOX4 axis which regulates the transcriptional activity of Anillin. Findings above demonstrate us a probable axis for HCC diagnosis and treatment. SUMMARY OF THE MAIN POINT: Anillin facilitates the cell proliferation and enhances tumor growth in HCC. The transcriptional activity of Anillin is modulated by miR-138/SOX4 axis. Findings above demonstrate us a probable axis for HCC diagnosis and treatment.

Coronavirus (COVID-19) combat: The power of chemistry

The pandemic of the Coronavirus Disease 2019 (COVID-19) has spread around the world. COVID-19 is one of the most serious infectious disease threats in the past 100 years. Epidemiological and clinical studies have shown that early diagnosis of infectious COVID-19 cases is of great help to slow down the spread of disease and reduce mortality. Therefore, the diagnosis of SARS-CoV-2 infection is of great significance in the control of the pandemic. However, because patients with COVID-19 show partially similar symptoms to diseases such as influenza-like illness, it is impossible to diagnose SARS-CoV-2 infection by relying solely on the patient's clinical features. Meanwhile, there are a number of asymptomatic patients, who are capable of spreading the infection while passing general surveillance. The early diagnosis of such cases is also critical for the prevention of the outbreak and control of the pandemic. Given there is no effective SARS-CoV-2-specific anti-viral agents, chemists are engaging in the development of analytical strategies for the SARS-CoV-2 detection and antiviral drug to combat the spread of the COVID-19. In order to support the innovation in the ongoing research on fighting COVID-19 pandemic, we summarized part of the achievements in SARS-CoV-2 detection and drug development engaged by chemists in China. By summarizing the available products and patents with practical potentials in fast analysis, we discussed the recent advances in the techniques for SARS-CoV-2 detection and their application potentials in diagnosis of COVID-19. For the diagnosis of COVID-19, the most specific analysis method is polymerase chain reaction (PCR) based nucleic acid assay. At the end of March, National Medical Products Administration has approved more than 20 products aiming at SARS-CoV-2 test, most of which are kits based on the principle of real-time quantitative reverse transcription PCR (RT-qPCR). Because PCR-based method needs complex manual operation steps, the technique is limited due to the time-consuming reaction steps. Later on, techniques with multiple procedures including nucleic acid extraction, amplification, and virus detection have been integrated for rapid detection. In order to solve the problems caused by disadvantages of complex thermal cycling process in PCR, isothermal amplification has been applied for nucleic acid detection. Combined with isothermal amplification, rapid point-of-care test is applicable for the detection of collected SARS-CoV-2 viral RNA. Although viral RNA assay is the most specific method for diagnosis of COVID-19, it cannot be used to determine the post-infection or monitor the immunity of general populations. The immunological assays could be applied for the detection of antibodies themselves several days after the infection by SARS-CoV-2. Therefore, lateral flow assay, microfluidics, and other technologies have successfully applied for the immune assay. Some reagents, detection kits, and devices have been certificated and marketed internationally. Specific anti-SARS-CoV-2 agents have been designed and tested. Moreover, several small-molecule drug candidates with high efficacy and low toxicity showed great potential in clinical application. Facing the outbreak of epidemic of COVID-19, Department of Chemical Sciences, Department of Mathematical and Physical Sciences of National Natural Science Foundation of China have jointly funded three innovative projects to support the research on the effective and expedite control of the epidemic. These projects include "Research on rapid detection of new coronavirus in aerosol on site", "A pre-clinical study of using small molecules to treat SARS-CoV-2-induced excessive inflammation and injury", and "Polymer micro-/nano-fibrous non-woven fabrics prepared via flash-pressure-released technology and reusable high-quality medical protective suits produced with these fabrics". The pandemic of COVID-19 is a major public health emergency, which poses a major challenge to health system worldwide and also has a m jor impact on economy and society. In response to sudden large-scale outbreaks, rapid, accurate and high-throughput detection technology is undoubtedly the priority of "anti-epidemic". Focusing on the present and serving the long-term requirement, we still need to innovatively propose new ideas to meet the major needs in the diagnosis and treatment of major diseases based on a global perspective.